routine laboratory test results, imaging results, and results of  Histo- plasma  antigen EIA on urine, if performed.
|2.3 Histoplasma  antigen detection in urine using enzyme immunoassay
|Results for  Histoplasma  antigen detection in urine (MVista  Histoplasma Antigen EIA, MiraVista Diagnostics, Indianapolis, IN) at the time of diagnosis were reviewed for animals with histoplasmosis. The report- able concentration was 0.4 to 19.0 ng/mL. For statistical analysis, detection of  Histoplasma  antigen but below or above the limit of quantification was considered to be 0.4 and 19.0 ng/mL, respectively. 2.4 Anti- Histoplasma  IgG detection in serum using enzyme immunoassay
|Commercially available laboratory-developed EIAs (MVista  Histo- plasma  Feline or Canine IgG EIA, MiraVista Diagnostics, Indianapolis, IN) with slight modifications to previously reported protocols were used. Ninety-six-well microplates were coated with proprietary Histoplasma  antigens and blocking buffer. Patient sera, standards, and controls were added to wells. After incubation at 37 C for 1 hour, wells were washed, and biotinylated anti-canine or anti-feline IgG was added to each well. The microplate was incubated at 37 C for 1 hour. The wells were washed, and streptavidin-horse radish peroxidase con- jugate was added to each well and then incubated at 37 C for 1 hour. The wells were washed, and chromogen solution containing peroxi- dase substrate was added and incubated at room temperature for 10 minutes. The enzymatic reaction was stopped  The word "con-" should not appear in your response.
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