```Axo-glial interactions contribute to synaptic morphogenesis and plasticity. However, the identify of all the axo-glial ligand-receptor interactions of neurohypophysis (NH), a major neuroendocrine interface has not been addressed. The NH-projecting axonal tracts, NH axo-glial cellular components and their ultrastructure are conserved in zebrafish. We aimed to identify the axo-glial interactome of Oxytocin (OXT) neurons and glial pituicytes. Due to the lack of OXT neuronal axonal transcriptome, we used the OXT neuron scRNA-seq data and glial pituicyte bulk RNA-seq data (Figure 4C). Using the differentially expressed list of genes from these datasets, we identified only 3 ligand-receptor interactions between OXT neurons and glial pituicytes at high confidence (Nts-Sort1a, Bdnf-Ngrfb/Sort1a) (Supplementary Figure S3C-E & Supplementary Table 10). To incorporate all the genes expressed in OXT neurons, we used the pseudo-bulk expression data of OXT neuron enriched cluster. This led to the identification of 259 ligands and 209 receptors in OXT neurons. Whereas in glial pituicytes transcriptome, we identified 325 ligands and 174 receptors with average read counts >50 (Supplementary Figure S3F-G & Supplementary Table 11). Analyzing the ligand-receptor pairs in this dataset led to identification of 37 OXT-neuron-glial pituicyte ligand-receptor interactions. The top ranked pituicyte ligand-OXT neuron receptor interactions were Slit3-Robo2/Robo1, Lama4-Dag1 and Vegfab-Nrp1a. While the top ranked OXT neuron ligand-pituicyte receptors were Mif-Cd74a, Nts-Sort1a/Sort1b, and Bdnf-Sort1a/Sort1b (Figure 4D & Supplementary Table 11). We also observed several autocrine signaling interactions in glial pituicytes (Cxcl12a-Cxcr4b, Tnfb-Tnfrsf1a, and Fgf8a/Fgf10a-Fgfr2) and in OXT neurons (Slit3/Slit2-Robo2/Robo1/Robo3, Bdnf-Ntrk3a/Sort1a, and Agrn-Dag1) (Figure 4D & Supplemental Table 11).``` - rephrase this The word "gene" should not appear in your response.
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