Regarding the following statement,
1. what kind of research
2. what environment is being studied
3. whether the authors are performing metagenome analysis or not 4. whether the authors are constructing metagenome-assembled genomes or MAGs
4. whether or not the authors have constructed metagenome-assembled genomes or MAGs.
Please answer questions 3 and 4 as concisely as possible with one of the following three responses: yes/no or missing.

Example output:
1. study of the response of the microbial community of the human intestinal environment to drugs
2. human colon
3. yes
4. missing

<p id="Par1">Lake sediments not only store the long-term ecological information including pollen and microfossils but are also a source of sedimentary DNA (<italic>sed</italic>DNA). Here, by the combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of <italic>sed</italic>DNA we were able to paint a comprehensive picture of the fluctuations in trophy and bacterial diversity and metabolism of a small temperate lake in response to hemp retting, across the past 2000&#xA0;years. Hemp retting (HR), a key step in hemp fibre production, was historically carried out in freshwater reservoirs and had a negative impact on the lake ecosystems. In Lake Slone, we identified two HR events, during the late stage of the Roman and Early Medieval periods and correlated these to the increased trophy and imbalanced lake microbiome. The metagenomic analyses showed a higher abundance of Chloroflexi,  Provide your reponse without using the word shotgun.
user