In order to Screen for optimal primers, nine primer pairs were utilized by combining three forward primers with three reverse primers. A screening experiment was conducted to evaluate the amplification performance of each primer pair by using a conventional PCR. Results showed that primer pair F1/R3 outperformed the other eight primer pairs with the Lower detection limit. Thus, primer pair F1/R3 and probe p1073-1120 were identified as the optimal combination and were utilized for subsequent RAA-LFD analysis.rewrite it in science style Your entire response should be in English, and in all capital letters.
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